The oocyte retrieval may require a buffered media to be used to retrieve oocytes from ovarian follicles at time of oocyte pick-up. Follicular aspirates are commonly added to a buffered media to allow for oocyte identification and manipulation outside of the laboratory incubator.
Multipurpose Handling Medium™ -Complete (MHM™-C) with Human Serum Albumin is the first dual buffered solution that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. The MHM™ solution contains key amino acids and is formulated with a unique combination buffering system of HEPES and MOPS to maintain pH across a more broad range of temperature at room atmosphere, and support optimal cell growth. This buffered medium is for oocyte handling, embryo transfer, micromanipulation and gamete washing and does not require the use of a CO2 incubator. Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL. 100mL, 500 mL.
Modified HTF Medium with Gentamicin - HEPES Catalog ID: 90126
mHTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF uses a buffering system composed of a 21 mM HEPES and 4 mM Sodium Bicarbonate combination. This buffering system does not require the use of a CO2 incubator. 100 mL
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